The 2025/26 Southern Region Women’s Football League Division One South will line up as follows:
- AFC Stoneham
- AFC Varsity
- Andover New Street (Promoted)
- Bemerton Heath Harlequins (Promoted)
- Bournemouth Sports Reserves
- Bursledon
- Farnham Town (Promoted)
- Guernsey (Joining from JFA Combination League)
- Havant & Waterlooville
- Nursling & Shirley (was QK Southampton)
- Shaftesbury
- Wimborne Town