Subject to any appeals to The Football Association, the 2025/26 Southern League Premier Division South will line up as follows:
- Basingstoke Town
- Berkhamsted (Promoted)
- Bracknell Town
- Chertsey Town
- Dorchester Town
- Evesham United (Promoted)
- Farnham Town (Promoted)
- Gloucester City
- Gosport Borough
- Hanwell Town
- Havant and Waterlooville
- Hungerford Town
- Plymouth Parkway
- Poole Town
- Sholing
- Taunton Town
- Tiverton Town
- Uxbridge (Promoted)
- Walton & Hersham
- Weymouth (Relegated)
- Wimborne Town
- Yate Town